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課程名稱 |
功能性Non-Coding RNA新知
Frontiers in Functional Non-coding Rnas |
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開課學期 |
111-2 |
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授課對象 |
生物資源暨農學院 生物科技研究所 |
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授課教師 |
林劭品 |
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課號 |
Biot8014 |
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課程識別碼 |
642ED0160 |
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班次 |
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學分 |
2.0 |
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全/半年 |
半年 |
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必/選修 |
選修 |
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上課時間 |
星期三6,7(13:20~15:10) |
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上課地點 |
生技所415 |
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備註 |
本課程以英語授課。
總人數上限：10人 |
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課程簡介影片 |
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核心能力關聯 |
核心能力與課程規劃關聯圖 |
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課程大綱
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課程概述 |
It is now clear that lots of non-coding RNAs in the genome are functional and very powerful in regulating gene activities and developmental process.
Depends on the background of the students, around 1/3 of the lectures will be given by the instructor for introducing basic concepts in non-coding RNA regulated gene silencing, including the post-transcriptional regulation by siRNA, microRNA and reproductive organ specific piRNAs. These will cover the physiological process including Genomic Imprinting, Sex Chromosome Compensation, Silencing of Endogenous Retrotransposons and Stem Cell Biology. In addition, the involvement of non-coding RNA related Regulatory Mechanisms in Carcinogenesis will be discussed.
For the rest of the classes, students will be assigned the most updated original research papers and conference articles as well as the milestone publications. They will be randomly divided into 2 groups for each assignment. One group will be presenting the articles and students in the other group are expected to challenge the papers from the experimental design, the methodology to the interpretation of the data. Students in the 1st group should defend for the papers as if it’s their manuscript. Students from the 2nd group should also provide constructive advice as how to improve the manuscript so that it can be published in a higher impact journal. |
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課程目標 |
On top of learning the basic concepts of non-coding RNA related Regulatory Mechanisms, keeping abreast of the most updated research internationally, students are expected to develop the ability to critically evaluate and interpret published research articles. In addition, they will learn the art of reviewing as well as defending a manuscript for publication. |
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課程要求 |
Preferably having studied one of the following subjects: (Introductory) Epigenetics, Special Topics in Reprogramming, Genetics and Epigenetics in Germ and Stem Cells |
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預期每週課後學習時數 |
1-4 hours from different phase of the program and also depending on students' prior knowledge of the subject |
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Office Hours |
另約時間 |
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指定閱讀 |
Statello, L., Guo, CJ., Chen, LL. et al. Gene regulation by long non-coding RNAs and its biological functions. Nat Rev Mol Cell Biol 22, 96–118 (2021). https://doi.org/10.1038/s41580-020-00315-9
Mattick, J.S., Amaral, P.P., Carninci, P. et al. Long non-coding RNAs: definitions, functions, challenges and recommendations. Nat Rev Mol Cell Biol (2023). https://doi.org/10.1038/s41580-022-00566-8
Candidate microRNAs as prognostic biomarkers in heart failure: A systematic review.
Figueiredo R, Adão R, Leite-Moreira AF, Mâncio J, Brás-Silva C.
Rev Port Cardiol. 2022 Oct;41(10):865-885. doi: 10.1016/j.repc.2021.03.020. Epub 2022 Aug 3.
Modern Methods for Assessment of microRNAs.
Bodulev OL, Sakharov IY.
Biochemistry (Mosc). 2022 May;87(5):425-442. doi: 10.1134/S0006297922050042.
PMID: 35790375 Review.
Circulating microRNAs in Medicine.
Pozniak T, Shcharbin D, Bryszewska M.
Int J Mol Sci. 2022 Apr 3;23(7):3996. doi: 10.3390/ijms23073996.
PMID: 35409354 Free PMC article. Review.
An overview of microRNAs: Biology, functions, therapeutics, and analysis methods.
Saliminejad K, Khorram Khorshid HR, Soleymani Fard S, Ghaffari SH.
J Cell Physiol. 2019 May;234(5):5451-5465. doi: 10.1002/jcp.27486. Epub 2018 Nov 23.
PMID: 30471116 Review.
piRNAs in sperm function and embryo viability.
Perillo G, Shibata K, Wu PH.
Reproduction. 2023 Jan 17;165(3):R91-R102. doi: 10.1530/REP-22-0312. Print 2023 Mar 1.
PMID: 36538648 Review.
piRNAs and PIWI proteins: regulators of gene expression in development and stem cells.
Rojas-Ríos P, Simonelig M.
Development. 2018 Sep 7;145(17):dev161786. doi: 10.1242/dev.161786.
PMID: 30194260 Review.
piRNAs in Gastric Cancer: A New Approach Towards Translational Research.
Fonseca Cabral G, Azevedo Dos Santos Pinheiro J, Vidal AF, Santos S, Ribeiro-Dos-Santos Â.
Int J Mol Sci. 2020 Mar 19;21(6):2126. doi: 10.3390/ijms21062126.
PMID: 32204558 Free PMC article. Review.
piRNAs: biogenesis and their potential roles in cancer.
Guo B, Li D, Du L, Zhu X.
Cancer Metastasis Rev. 2020 Jun;39(2):567-575. doi: 10.1007/s10555-020-09863-0.
PMID: 31960205 Review.
Regulation of alternative splicing by snoRNAs.
Kishore S, Stamm S.
Cold Spring Harb Symp Quant Biol. 2006;71:329-34. doi: 10.1101/sqb.2006.71.024.
PMID: 17381313 Review.
Regulation of alternative splicing by snoRNAs.
Kishore S, Stamm S.
Cold Spring Harb Symp Quant Biol. 2006;71:329-34. doi: 10.1101/sqb.2006.71.024.
PMID: 17381313 Review. |
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參考書目 |
Updated journal articles and reviews; “Epigenetics: Concepts, Theories, Paradigms and Mechanisms” and “Stem cell and Regenerative Medicine” CD-Roms (A Henry StewartTalks Series in the Life Sciences), National and International Conference Abstracts and Papers. Unpublished data. |
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評量方式
(僅供參考) |
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No. |
項目 |
百分比 |
說明 |
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1. |
Discussion over classes (of pre- / post- class study materials) |
30% |
Group and/or individual scores |
2. |
Open Book Midterm Exam |
20% |
Individual score, basic comprehension of key words |
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Oral Presentations |
25% |
Individual or Group presentations over relatively recent review articles or research publications |
4. |
Final Written Reports |
25% |
Up to 5 pages (including Figures/Tables and references) of written report in the format of one of the following: News and Views; mini-review; Pre-proposal; reviewer's comments format. |
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針對學生困難提供學生調整方式 |
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上課形式 |
以錄影輔助, 提供學生彈性出席課程方式 |
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作業繳交方式 |
延長作業繳交期限, 書面報告取代口頭報告, 個人報告取代團體報告, 學生與授課老師協議改以其他形式呈現 |
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考試形式 |
書面(口頭)報告取代考試 |
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其他 |
由師生雙方議定 |
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週次 |
日期 |
單元主題 |
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第1週 |
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Scope of functional RNAs |
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第2週 |
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Examples of functional long (non-coding) RNAs/ Brief introduction of epigenomics
https://www.google.com/search?q=ameba+sister+and+epigenetics&rlz=1C5CHFA_enTW990TW991&oq=ameba&aqs=chrome.2.69i57j35i39l2j0i512l5j46i10i199i433i465i512j0i10i131i433i512.4227j0j7&sourceid=chrome&ie=UTF-8#fpstate=ive&vld=cid:aa9b2d81,vid:MD3Fc0XOjWk
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第3週 |
2023/3/8 |
Preparation of the 1-slide/ 3 minute presentation about the importance of functional long (non-coding) RNAs (supplementary videos and review articles provided) |
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第4週 |
2023/3/15 |
Students' showtime trial 1.0 (3 mins/group):
The significance of functional (non-coding) RNAs |
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第5週 |
2023/3/22 |
microRNAs: from mechanisms to potentials as biomarkers/therapeutic targets
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第6週 |
2023/3/29 |
Students' showtime trial 2.0 (3 mins/group):
Real-life example of microRNAs' function of diagnosis/therapeutic power |
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第7週 |
2023/4/5 |
Spring break |
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第8週 |
2023/4/12 |
Open book midterm exam |
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第9週 |
2023/4/19 |
PIWI-interacting RNAs (piRNAs): Modulating transposable elements and beyond |
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第10週 |
2023/4/26 |
Students' showtime trial 3.0 (3 mins/group):
The significance of piRNAs in germ cells, neural cells or cancer cells |
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第11週 |
2023/5/3 |
SnoRNAs and other functional non-coding RNAs/
Tips for reading / presenting/ evaluating original research articles |
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第12週 |
2023/5/10 |
Preparation for journal article presentation/evaluation |
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第13週 |
2023/5/17 |
Author/Reviewer role playing Part I:
Original research article presentation and critical evaluation |
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第14週 |
2023/5/24 |
Author/Reviewer role playing Part II:
Original research article presentation and critical evaluation |
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第15週 |
2023/5/31 |
Author/Reviewer role playing Part III:
Original research article presentation and critical evaluation |
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第16週 |
2023/6/7 |
Written report due (One of the following formats: News and Views, Minireview, Reviewers' comments; Proposal) 5% daily deduction for delayed reports |
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第17週 |
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Extended proactive learning and bonus opportunities |
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第18週 |
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Extended proactive learning and bonus opportunities |
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